In order to successfully combined in vivo 2-photon GCaMP6f imagining and red retrobeads, I need to know the 2-photon excitation spectrum for the beads. I couldn’t find the information online, and the lumafluor didn’t have it, so I produced a rough and ready estimate. First, the power of the laser was measured in the range of 740 to 940 nm, then a small sample of undiluted retrobeads was held in the bottom of a sealed capillary tube, and the mean pixel value of the center of this capillary was measured as the wavelength was changed. I have presented the data as both the raw pixel values (Arb.) and the pixel values normalized to the power at the sample (Arb/µW). Long story short: between 780 and 880 nm is where you want to image.
EDIT: I have adjusted the axis as per the comment. I was just going to use “brightness”, but more descriptive = more better, right?